A novel substrate for in situ synthesis of oligonucleotide: plasma-treated polypropylene microporous membrane

作者：Tang JX; He NY^*; Tan MJ; He QG; Chen H

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2004, 242(1-3): 53-60.

DOI：10.1016/j.colsurfa.2004.04.054

• plasma
• grafting
• amino group
• oligonucleotide
• DMT solution

Polypropylene microporous membrane was treated with plasma in a mixture of nitrogen and hydrogen (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstrated the success of amino functional groups grafting. The density of polar amino groups on the membrane surface is approximately 0.5 mumol/cm(2), which is much higher than that of amino functional groups on modified glass slides. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield of more than 98% was achieved. These modified microporous membranes open novel prospects for the in situ synthesis of DNA microarrays.

• 出版日期2004-8-2
• 单位东南大学