Multiple cytokine analysis in human tears: An optimized procedure for cytometric bead-based assay

作者：LaFrance Martin W; Kehinde Lucy E; Fullard Roderick J^*

来源：Current Eye Research, 2008, 33(7): 525-544.

DOI：10.1080/02713680802190085

• cytokine
• immunoassay
• Luminex
• tear
• validation

Purpose: To compare instruments and reagents used for cytometric bead-based assay (CBA) of cytokines in human tears. Methods: Two CBA instruments and reagent kits from five vendors were compared in parallel. Vendor protocols were modified to accommodate tear volumes. Precision, recovery, dilution linearity, and stability were evaluated. Results: Luminex(TM) and Bio-Rad proved optimal for tear CBA. Good correlation for dilution linearity was observed (r > 0.80, p > 0.05) for 1-4-mu l sample volumes. Samples were stable for 30 min at 25 degrees C. Conclusion: Many tear cytokines can be reliably measured by CBA using an optimized protocol. Concentrations remain stable during periods consistent with collection.

• 出版日期2008