Antigen-Specific Transfer of Functional Programmed Death Ligand 1 from Human APCs onto CD8(+) T Cells via Trogocytosis

作者：Gary Regina; Voelkl Simon; Palmisano Ralf; Ullrich Evelyn; Bosch Jacobus J; Mackensen Andreas^*

来源：The Journal of Immunology, 2012, 188(2): 744-752.

DOI：10.4049/jimmunol.1101412

Upon specific interaction with APCs, T cells capture membrane fragments and surface molecules in a process termed trogocytosis. In this study, we demonstrate that human Ag-specific CD8(+) T cells acquire the coinhibitory molecule programmed death ligand 1 (PD-L1) from mature dendritic cells (mDC) and tumor cells in an Ag-specific manner. Immature dendritic cells were less effective in transferring surface molecules onto CD8(+) T cells than mDCs. Interestingly, trogocytosis of PD-L1 requires cell-cell contact and cannot be induced by uptake of soluble proteins obtained from mDC lysates. The transfer process is impaired by inhibition of vacuolar ATPases in T cells as well as by fixation of dendritic cells. Of importance, CD8(+) T cells that acquired PD-L1 complexes were able to induce apoptosis of neighboring programmed death 1-expressing CD8(+) T cells. In summary, our data demonstrate that human CD8(+) T cells take up functionally active PD-L1 from APCs in an Ag-specific fashion, leading to fratricide of programmed death 1-expressing, neighboring T cells. The transfer of functionally active coinhibitory molecules from APCs onto human CD8(+) T cells could have a regulatory role in immune responses. The Journal of Immunology, 2012, 188: 744-752.

• 出版日期2012-1-15