Apo-10 '-lycopenoic acid inhibits cancer cell migration and angiogenesis and induces peroxisome proliferator-activated receptor gamma

作者：Cheng Junrui; Miao Benchun; Hu Kang Quan; Fu Xueyan; Wang Xiang Dong^*

来源：Journal of Nutritional Biochemistry, 2018, 56: 26-34.

DOI：10.1016/j.jnutbio.2018.01.003

• Lycopene metabolites
• PPAR gamma
• Angiogenesis
• Cell migration
• Metastasis

Scope: We have previously shown that apo-10'-lycopenoic acid (ALA), a derivative of lycopene through cleavage by carotene-9',10'-oxygenase, inhibits tumor progression and metastasis in both liver and lung cancer animal models. The underlying mechanism remains unknown. We hypothesized that ALA inhibits cancer cell motility and angiogenesis by up-regulating peroxisome proliferator-activated receptor gamma (PPAR gamma) which is involved in controlling angiogenesis, tumor progression and metastasis.
Methods and results: ALA treatment, in dose-dependent manner, was effective at inhibiting migration and invasion of liver and lung cancer cells (HuH7 and A549) in both Transwell and wound-healing models, as well as suppressing actin remodeling and ruffling/lamellipodia formation in HuH7 and immortalized lung BEAS-2B cells. ALA treatment resulted in suppression of angiogenesis in both tube formation and aortic ring assays and inhibition of matrix metalloproteinase-2 expression and activation in both HuH7 and A549 cells. Additionally, ALA dose-dependently increased the mRNA expression and protein levels of PPAR gamma in human THLE-2 liver cells.
Conclusion: ALA inhibits cancer cell motility and angiogenesis and induces PPAR gamma expression, which could be one of the potential mechanisms for ALA protecting against tumor progression.

• 出版日期2018-6