MicroRNA-155 modulates the interleukin-1 signaling pathway in activated human monocyte-derived dendritic cells

作者：Ceppi Maurizio; Pereira Patricia M; Dunand Sauthier Isabelle; Barras Emmanuele; Reith Walter; Santos Manuel A; Pierre Philippe^*

来源：Proceedings of the National Academy of Sciences, 2009, 106(8): 2735-2740.

DOI：10.1073/pnas.0811073106

• bic/miR-155
• LPS
• TAB2
• TLR/IL-1 pathway

In response to inflammatory stimulation, dendritic cells (DCs) have a remarkable pattern of differentiation (maturation) that exhibits specific mechanisms to control immunity. Here, we show that in response to Lipopolysaccharides (LPS), several microRNAs (miRNAs) are regulated in human monocyte-derived dendritic cells. Among these miRNAs, miR-155 is highly up-regulated during maturation. Using LNA silencing combined to microarray technology, we have identified the Toll-like receptor/interleukin-1 (TLR/IL-1) inflammatory pathway as a general target of miR-155. We further demonstrate that miR-155 directly controls the level of TAB2, an important signal transduction molecule. Our observations suggest, therefore, that in mature human DCs, miR-155 is part of a negative feedback loop, which down-modulates inflammatory cytokine production in response to microbial stimuli.

• 出版日期2009-2-24