Molecular-Scale Force Measurement in a Coiled-Coil Peptide Dimer by Electron Spin Resonance

作者：Gulla Stefano V; Sharma Gaurav; Borbat Peter; Freed Jack H; Ghimire Harishchandra; Benedikt Monica R; Holt Natasha L; Lorigan Gary A; Rege Kaushal; Mavroidis Constantinos; Budil David E^*

来源：Journal of the American Chemical Society, 2009, 131(15): 5374-+.

DOI：10.1021/ja900230w

A new method for measuring forces between small protein domains based on double electron-electron resonance (DEER) spectroscopy is demonstrated using a model peptide derived from the alpha-helical coiled-coil leucine zipper of yeast transcriptional activator GCN4. The equilibrium distribution of distances between two nitroxide spin labels rigidly attached to the helices of the dimer was determined by DEER and yielded a closing force of 100 /- 10 pN between monomers, in excellent agreement with theoretical predictions.

• 出版日期2009-4-22
• 单位东北大学