Molecular marker-based characterization and genetic diversity of wheat genotypes in relation to Boron use efficiency

作者:Emon R M*; Gustafson J P; Nguyens H; Musket T; Jahiruddin M; Islam M A; Haque M S; Islam M M; Begum S N; Hassan M M
来源:Indian Journal of Genetics and Plant Breeding, 2010, 70(4): 339-348.

摘要

Boron deficient soils pose a serious problem to world wheat production including Bangladesh. In the present study, 21 diverse wheat (Triticum aestivum L.) genotypes were subjected to SSR analysis to identify and characterize boron efficient varieties. In the DNA profiling of the 21 genotypes, Including two control varieties, Fang 60 (efficient) and SW 41 (inefficient), using 32 SSR loci, a total of 234 alleles were detected. Allele number per locus ranged from 3 to 11, with an average of 7.312, and the PIC value ranged from 0.562 to 0.873 with an average of 0.776. Average genetic diversity over all SSR loci for the 21 genotypes was 0.804, ranging from 0.637 to 0.884. All the loci were polymorphic and clearly distinguished the genotypes. Cluster analyse (NJ tree, UPGMA, PCO) identified a similar pattern of variation. The study found that INIA 66 and BAW1086 were the most boron efficient genotypes and thus could be used for developing boron efficient varieties.

  • 出版日期2010-11