Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3 beta and APC and Inhibiting Gene beta-Catenin

作者：Xu Ruoshi; Kang Qiumei; Ren Jie; Li Zukun; Xu Xiaoping^*

来源：Journal of Analytical Methods in Chemistry, 2013, 2013: 1-7.

DOI：10.1155/2013/951319

Objective. Inhibiting gene beta-catenin and inducting genes GSK-3 beta and APC, promoting the tumor cell apoptosis in Wnt pathway, by chlorogenic acid were discussed (CGA). Method. The different genes were scanned by the 4* 44K mouse microarray chips. The effect of the three genes was confirmed by RT-PCR technique with CGA dosage of 5, 10, and 20 mg/kg. Result. The expression of GSK-3 beta and APC was upregulated in group of 20 mg/kg dosage (P < 0.05) and the expression of beta-catenin was downregulated in the same dosage (P < 0.05). Conclusion. The results infer that the multimeric protein complex of beta-catenin could be increased by CGA upregulated genes GSK-3 beta and APC, which could inhibit the free beta-catenin into the nucleus to connect with TCF. So the transcriptional expression of the target genes will be cut to abnormal cell proliferation. It is probably one of the ways that can stop the tumor increase by CGA.

• 出版日期2013
• 单位四川大学