Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase

作者：Lo Leggio Leila; Simmons Thomas J; Poulsen Jens Christian N; Frandsen Kristian E H; Hemsworth Glyn R; Stringer Mary A; von Freiesleben Pernille; Tovborg Morten; Johansen Katja S; De Maria Leonardo; Harris Paul V; Soong Chee Leong; Dupree Paul; Tryfona Theodora; Lenfant Nicolas; Henrissat Bernard; Davies Gideon J; Walton Paul H^*

来源：Nature Communications, 2015, 6(1): 5961.

DOI：10.1038/ncomms6961

Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by beta-amylase. The detailed structure of the enzyme's active site yields insights into the mechanism of action of this important class of enzymes.

• 出版日期2015-1