A whole-genome bioinformatics approach to selection of antigens for systematic antibody generation

作者：Berglund Lisa; Bjorling Erik; Jonasson Kalle; Rockberg Johan; Fagerberg Linn; Szigyarto Cristina Al Khalili; Sivertsson Asa; Uhlen Mathias^*

来源：Proteomics, 2008, 8(14): 2832-2839.

DOI：10.1002/pmic.200800203

• antibody generation
• antigen selection
• bioinformatics
• epitope
• sequence similarity

Here, we present an antigen selection strategy based on a whole-genome bioinformatics approach, which is facilitated by an interactive visualization tool displaying protein features from both public resources and in-house generated data. The web-based bioinformatics platform has been designed for selection of multiple, non-overlapping recombinant protein epitope signature tags by display of predicted information relevant for antigens, including domain- and epitope sized sequence similarities to other proteins, transmembrane regions and signal peptides. The visualization tool also displays shared and exclusive protein regions for genes with multiple splice variants. A genome-wide analysis demonstrates that antigens for approximately 80% of the human protein-coding genes can be selected with this strategy.

• 出版日期2008-7