A nucleic acid probe labeled with desmethyl thiazole orange: a new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging

作者：Okamoto Akimitsu^*; Sugizaki Kaori; Yuki Mizue; Yanagisawa Hiroyuki; Ikeda Shuji; Sueoka Takuma; Hayashi Gosuke; Wang Dan Ohtan

来源：Organic and Biomolecular Chemistry, 2013, 11(2): 362-371.

DOI：10.1039/c2ob26707a

A new fluorescent nucleotide with desmethyl thiazole orange dyes, D-505', has been developed for expansion of the function of fluorescent probes for live-cell RNA imaging. The nucleoside unit of D-505' for DNA autosynthesis was soluble in organic solvents, which made the preparation of nucleoside units and the reactions in the cycles of DNA synthesis more efficient. The dyes of D-505'-containing oligodeoxynucleotide were protonated below pH 7 and the oligodeoxynucleotide exhibited hybridization-sensitive fluorescence emission through the control of excitonic interactions of the dyes of D-505'. The simplified procedure and effective hybridization-sensitive fluorescence emission produced multicolored hybridization-sensitive fluorescent probes, which were useful for live-cell RNA imaging. The acceptor-bleaching method gave us information on RNA in a specific cell among many living cells.

• 出版日期2013