A rapid separation and determination of amino acids by capillary electrophoresis with a high frequency conductivity detector

作者：Zhai, HY; Cai, PX; Chen, ZG^*; Li, GH; Ye, X; Mo, JY

来源：Chemical Journal of Chinese Universities, 2004, 25(6): 1037-1039.

• reversed capillary electrophoresis
• high frequency conductivity detection
• contactless conductivity detection
• amino acids

A reversed capillary electrophoresis with contactless high frequency conductivity detection method to determine amino acids mixture was described. In this study, cetyltrimethylammonium bromide (CTAB) was used-to reverse electro-osmotic flow in order to shorten the analysis time. beta-CD was added to optimize the separation further. The. effects of separation potential, concentration of CTAB, buffer electrolyte and beta-CD etc. were researched and the best analysis conditions were obtained. Ten kinds of amino acids ware separated and detected directly in running medium of 15.0 mmol/L diethylamine/0.10 mmol/L H3BO3/0.10 mmol/L CTAB/0.25 mmol/L beta-CD (pH = 11.0) at the separation potential of -20 kV within 7 min. The correlation coefficients of amino acids are 0.991-0.999 in the linear range of 1.00 x 10(-6)-1.00 x 10(-3) mol/L, the limit of detection are 0.17-0-94 mumol/L (S/N = 3).

• 出版日期2004-6-10
• 单位中山大学