Endothelin-1 shifts the mediator of bradykinin-induced relaxation from NO to H2O2 in resistance arteries from patients with cardiovascular disease

作者：Leurgans Thomas M; Bloksgaard Maria; Brewer Jonathan R; Bagatolli Luis A; Fredgart Maise H; Rosenstand Kristoffer; Hansen Maria L; Rasmussen Lars M; Irmukhamedov Akhmadjon; De Mey Jo G R^*

来源：British Journal of Pharmacology, 2016, 173(10): 1653-1664.

DOI：10.1111/bph.13467

Background and PurposeWe tested the hypothesis that in resistance arteries from cardiovascular disease (CVD) patients, effects of an endothelium-dependent vasodilator depend on the contractile stimulus.
Experimental ApproachArteries dissected from parietal pericardium of cardiothoracic surgery patients were studied by myography and imaging techniques. Segments were sub-maximally contracted by K+, the TxA(2) analogue U46619 or endothelin-1 (ET-1).
Key ResultsRelaxing effects of Na-nitroprusside were comparable, but those of bradykinin (BK) were bigger in the presence of ET-1 compared with K+ or U46619. BK-induced relaxation was (i) abolished by L-NAME in K+-contracted arteries, (ii) partly inhibited by L-NAME in the presence of U46619 and (iii) not altered by indomethacin, L-NAME plus inhibitors of small and intermediate conductance calcium-activated K+ channels, but attenuated by catalase, in ET-1-contracted arteries. This catalase-sensitive relaxation was unaffected by inhibitors of NADPH oxidases or allopurinol. Exogenous H2O2 caused a larger relaxation of ET-1-induced contractions than those evoked by K+ or U46619 in the presence of inhibitors of other endothelium-derived relaxing factors. Catalase-sensitive staining of cellular ROS with CellROX Deep Red was significantly increased in the presence of both 1M BK and 2nM ET-1 but not either peptide alone.
Conclusions and ImplicationsIn resistance arteries from patients with CVD, exogenous ET-1 shifts the mediator of relaxing responses to the endothelium-dependent vasodilator BK from NO to H2O2 and neither NADPH oxidases, xanthine oxidase nor NOS appear to be involved in this effect. This might have consequences for endothelial dysfunction in conditions where intra-arterial levels of ET-1 are enhanced.

• 出版日期2016-5