Mitochondrial peroxiredoxins are critical for the maintenance of redox state and the survival of adult Drosophila

作者：Radyuk Svetlana N; Rebrin Igor; Klichko Vladimir I; Sohal Barbara H; Michalak Katarzyna; Benes Judith; Sohal Rajindar S; Orr William C^*

来源：Free Radical Biology and Medicine, 2010, 49(12): 1892-1902.

DOI：10.1016/j.freeradbiomed.2010.09.014

• Peroxiredoxin
• Mitochondria
• Redox state
• Thiol
• Thioredoxin
• Glutathione
• Oxidative stress
• Apoptosis
• Aging
• Drosophila
• Free radicals

Drosophila mitochondria contain two peroxidases peroxiredoxin 3 (dPrx3) and peroxiredoxin 5 (dPrx5) which together constitute the sole known intramitochondrial mechanism for the catalytic removal of hydrogen and organic peroxides dPrx3 exists exclusively within mitochondria whereas dPrx5 is also present in some other intracellular compartments Levels of these two peroxiredoxins were genetically manipulated singly and together in D melanogaster for the purpose of understanding their respective functions Underexpression of dPrx3 by 90-95% had no discernable effect on life span under normal or oxidative stress conditions the dPrx5 null flies were previously reported to exhibit a 10% shortening of mean life span and an increase in sensitivity to oxidative stress Flies underexpressing both dPrx3 and dPrx5 showed an 80% decrease in life span a severe disruption in thiol homeostasis and a massive induction of apoptosis in the muscle and digestive system tissues The early mortality in flies underexpressing both peroxiredoxins was partially offset by overexpression of thioredoxin reductase but not mitochondrion-targeted catalase These results suggest that mitochondrial peroxiredoxins confer specific protection for th

• 出版日期2010-12-15