Detection of donor-specific antibodies in kidney transplantation

作者：Haarberg KelleyM K; Tambur Anat R^*

来源：British Medical Bulletin, 2014, 110(1): 23-34.

DOI：10.1093/bmb/ldu005

• C1q
• complement-binding antibodies
• donor-specific antibodies
• HLA-antibody epitope
• HLA-DQ antibodies
• prozone
• solid-phase assays

Precise and timely detection of human leukocyte antigen (HLA) donor-specific antibodies (DSAs) is vital for evaluating humoral immune status of patients pre- and post-transplantation.
Clinically relevant articles on theory, development, methodology and application of HLA-DSA testing in kidney transplantation.
The availability of solid phase HLA-antibody testing revolutionized our ability to detect HLA-DSA and to appreciate their significance in kidney transplant outcome. The best approach to determine the strength, immunogenicity and pathogenicity of HLA antibodies still remains controversial.
Assays to identify complement-binding antibodies were developed. Their clinical utilization, pre- and post-transplantation, is currently under investigation. Appreciation of the complexity of HLA-DQ antibodies should lead to better assignment of unacceptable antibodies and cPRA calculation.
Characterization of HLA-antibody epitopes, and utilization of epitope matching to better define compatible donors could contribute to better transplant outcomes.

• 出版日期2014-6
• 单位西北大学