Advanced bile acid-based multi-compartmental microencapsulated pancreatic beta-cells integrating a polyelectrolyte-bile acid formulation, for diabetes treatment

作者：Mooranian Armin; Negrulj Rebecca; Chen Tan Nigel; Fakhoury Marc; Arfuso Frank; Jones Franca; Al Salami Hani^*

来源：Artificial Cells, Nanomedicine and Biotechnology, 2016, 44(2): 588-595.

DOI：10.3109/21691401.2014.971806

• bile acid
• pancreatic beta cells
• Seahorse analyzer
• microencapsulation

This study utilized the Seahorse Analyzer to examine the effect of the bile acid ursodeoxycholic acid (UDCA), on the morphology, swelling, stability, and size of novel microencapsulated beta-cells, in real-time. UDCA was conjugated with fluorescent compounds, and its partitioning within the microcapsules was examined using confocal microscopy. UDCA produced microcapsules with good morphology, better mechanical strength (p < 0.01), and reduced swelling properties (p < 0.01), but lower cell viability (p < 0.05) and cell count per microcapsule (p < 0.01). UDCA reduced the cells' biochemical activities, mitochondrial respiration, and energy production, post-microencapsulation. This is the first time biological functions of microencapsulated beta-cells have been analyzed in real-time.

• 出版日期2016-2-17