Nucleotide sequence and chromosomal localization of the gene for pierisin-1, a DNA ADP-ribosylating protein, in the cabbage butterfly Pieris rapae

作者：Yamamoto Masafumi^*; Takahashi Nakaguchi Azusa; Matsushima Hibiya Yuko; Nakano Tsuyoshi; Totsuka Yukari; Imanishi Shigeo; Mitsuhashi Jun; Watanabe Masahiko; Nakagama Hitoshi; Sugimura Takashi; Wakabayashi Keiji

来源：Genetica, 2011, 139(10): 1251-1258.

DOI：10.1007/s10709-011-9626-5

• Fluorescence in situ hybridization
• Karyotypes
• Genomic structure
• Lepidoptera

Cabbage butterfly, Pieris rapae, contains a unique DNA ADP-ribosylating protein, pierisin-1, which transfers ADP-ribose moiety of NAD to guanine bases of DNA. Pierisin-like proteins are only distributed in subtribes Pierina, Aporiina and Appiadina of the family Pieridae. In this study, we obtained genomic clones carrying the pierisin-1 gene from adult samples of P. rapae by plaque hybridization. The pierisin-1 gene was found to consist of two exons, 0.1-kb exon 1 and 3.9-kb exon 2, and a 2.3-kb intron. In addition, we could demonstrate that the putative promoter in the about 3-kb upstream region from the transcription start site of the gene include a transcriptional activating motif involved in immune pathways and hormonal regulation. We also examined chromosomal localization of the pierisin-1 gene. Fluorescence in situ hybridization (FISH) analysis using Cy3-labeled pierisin-1 genomic clone demonstrated the localization of the gene near the kinetochore in chromosome 9. Thus, we confirmed that the pierisin-1 gene is located in the genome of P. rapae.

• 出版日期2011-10