Neuroprotective effect of mesenchymal stem cell through complement component 3 downregulation after transient focal cerebral ischemia in mice

作者：Jung Hye Seon; Jeong Si Yeon; Yang Jiwon; Kim So Dam; Zhang Baojin; Yoo Hyun Seung; Song Sun U; Jeon Myung Shin^*; Song Yun Seon^*

来源：Neuroscience Letters, 2016, 633: 227-234.

DOI：10.1016/j.neulet.2016.09.032

• Mesenchymal stem cell
• Complement component 3
• Neuroprotection
• Cerebral ischemia
• Oxygen glucose deprivation

Bone marrow-derived mesenchymal stem cells (MSCs) are used in stroke treatment despite the poor understanding of its mode of action. The immune suppressive and anti-inflammatory properties of MSCs possibly play important roles in regulating neuroinflammation after stroke. We investigated whether MSCs reduce the inflammatory complement component 3 (C3) levels, thus, providing neuroprotection during stroke. Mice were subjected to transient focal cerebral ischemia (tFCI), after which MSCs were intravenously injected. The infarct volume of the brain was reduced in MSC-injected tFCI mice, and C3 expression was significantly reduced in both the brain and the blood. Additionally, the profiles of other inflammatory mediators demonstrated neuroprotective changes in the MSCs-treated group. In order to analyze the effect of MSCs on neurons during cerebral ischemia, primary cortical neurons were co cultured with MSCs under oxygen-glucose deprivation (OGD). Primary neurons co -cultured with MSCs exhibited reduced levels of C3 expression and increased protection against OGD, indicating that treatment with MSCs reduces excessive C3 expression and rescues ischemia-induced neuronal damage. Our finding suggests that reduction of C3 expression by MSCs can help to ameliorate ischemic brain damage, offering a new neuroprotective strategy in stroke therapy.

• 出版日期2016-10-28
• 单位河北医科大学