Multianalyte immunoassay chip for detection of tumor markers by chemiluminescent and colorimetric methods

作者：Wei Wei; Zhang Chunyan; Qian Jing; Liu Songqin^*

来源：Analytical and Bioanalytical Chemistry, 2011, 401(10): 3269-3274.

DOI：10.1007/s00216-011-5400-x

• Multianalyte immunoassay
• Tumor markers
• Chemiluminescence
• Colorimetric method
• Chip

Most cancers developed an elevation of the level of at least two markers associated with their incidence. Simultaneous detection of multi-tumor markers associated with a particular type of cancer plays an important role in cancer diagnostic. Here, a multianalyte immunoassay chip for simple and sensitive detection of tumor markers with chemiluminescent and colorimetric methods was proposed, in which carcinoembryonic antigen (CEA) and carbohydrate antigen (CA19-9) that associated with colorectal cancer were detected as model. The immunoassay chip was fabricated by co-immobilization of CEA/CA19-9 antibody on a glass slide with gamma-glycidoxypropyltrimethoxysilane as linkage. Through sandwiched immunoreactions, CEA, CA19-9, and their corresponding enzyme tracers, alkaline phosphatase-labeled anti-CEA and horseradish peroxidase-labeled anti-CA19-9, were introduced on the chip. Then, they were sequentially detected by chemiluminescent method in the range of 0.5-80 mu g/L and 0.5-80 kU/L with the detection limits of 0.41 mu g/L and 0.36 kU/L at 3 sigma for CEA and CA19-9, respectively. They could also be detected by colorimetric method in the range of 1-200 mu g/L and 5-200 kU/L with the detection limits of 0.25 mu g/L and 1.25 kU/L at 3 sigma for CEA and CA19-9, respectively. All these results demonstrated that the present work provided a promising analytical method for tumor markers' analysis with the advantages of simple analytical procedure, small sample volume and lower cost, which made the proposed method potential for high-throughput detection.

• 出版日期2011-12
• 单位东南大学