Clinical microfluidics for neutrophil genomics and proteomics

作者：Kotz Kenneth T^*; Xiao Wenzong; Miller Graziano Carol; Qian Wei Jun; Russom Aman; Warner Elizabeth A; Moldawer Lyle L; De Asit; Bankey Paul E; Petritis Brianne O; Camp David G II; Rosenbach Alan E; Goverman Jeremy; Fagan Shawn P; Brownstein Bernard H; Irimia Daniel; Xu Weihong; Wilhelmy Julie; Mindrinos Michael N; Smith Richard D; Davis Ronald W; Tompkins Ronald G; Toner Mehmet

来源：Nature Medicine, 2010, 16(9): 1042-U142.

DOI：10.1038/nm.2205

Neutrophils have key roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood with 'on-chip' processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and by comparison with standard bulk isolation methodologies. Last, we implement this tool as part of a near-patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury. The preliminary results from a small cohort of subjects in our study and healthy controls show a unique time-dependent gene expression pattern clearly demonstrating the ability of this tool to discriminate temporal transcriptional events of neutrophils within a clinical setting.

• 出版日期2010-9