Hyaluronan suppresses mechanical stress-induced expression of catabolic enzymes by human chondrocytes via inhibition of IL-1 beta production and subsequent NF-kappa B activation

作者：Ozawa Masatsugu; Nishida Keiichiro^*; Yoshida Aki; Saito Taichi; Harada Ryozo; Machida Takahiro; Ozaki Toshifumi

来源：Inflammation Research, 2015, 64(3-4): 243-252.

DOI：10.1007/s00011-015-0804-2

• Chondrocyte
• Hyaluronan
• Mechanical stress
• Aggrecanase
• NF-kappa B
• RUNX-2

To investigate the inhibitory effect of hyaluronan (HA) on mechanical stress- induced expression of a disintegrin and metalloproteinase with thrombospondin type1 motifs (ADAMTS)-4, -5 and matrix metalloproteinase (MMP)-13 by human chondrocytes.
Normal human articular chondrocytes were pre-incubated with or without 1.0 mg/mL HA (2700 kDa) for 12 h at 37 A degrees C in stretch chambers, then they were exposed to uni-axial cyclic tensile strain (CTS, 0.5 Hz, 10 % elongation). The expression of ADAMTS-4, -5, and MMP-13 were analyzed by real-time polymerase chain reaction and Immunocytochemistry. The concentration of IL-1 beta in the supernatant was measured using enzyme-linked immunosorbent assay (ELISA). The nuclear translocation of runt-related transcription factor 2 (RUNX-2) and nuclear factor-kappa B (NF-kappa B) was examined by ELISA and immunocytochemistry, and phosphorylation of NF-kappa B was examined by western blotting.
HA inhibited mRNA expression of ADAMTS-4, -5, and MMP13 after 24 h CTS via inhibition of IL-1 beta secretion and NF-kappa B activation. However, HA failed to inhibit CTS-induced RUNX-2 expression and subsequent expression of ADAMTS-5 and MMP-13 1 h after CTS.
Our results demonstrated that HA significantly suppressed mechanical stress-induced expression of catabolic proteases by inhibition of the NF-kappa B-IL-1 beta pathway, but did not suppress mechanical stress-induced RUNX-2 signaling.

• 出版日期2015-4