Flagella and Pili of Xanthomonasaxonopodis pv. glycines are associated with motility, biofilm formation and virulence on soybean

作者：Athinuwat Dusit; Brooks Siraprapa; Burr Thomas J; Prathuangwong Sutruedee^*

来源：Journal of Phytopathology, 2018, 166(7-8): 590-600.

DOI：10.1111/jph.12722

• soybean disease
• swarming motility
• twitching motility

Targeted mutations in flgK, and pilD genes in strain KU-P-SW005 of Xanthomonasaxonopodis pv. glycines, the cause of pustule disease on soybean, led to altered motility phenotypes. The flgK mutants lacked a monopolar flagellum and lost swimming motility, whereas the pilD mutant lacked type IV pili and was unable to move via twitching, a form of surface motility not previously reported for this pathogen. The flgK and pilD mutants were also altered in biofilm production. The flgK and pilD mutants caused reduced disease in susceptible soybean cultivars Spencer when compared to KU-P-SW005. Cell counts of the flgK and pilD mutants on plants remained equivalent to KU-P-SW005 10days after inoculation. Complementation of flgK and pilD mutants restored all phenotypes to wild-type levels. Therefore, flgK and pilD genes that are required for swimming and twitching motility also affected biofilm formation and virulence on soybean.

• 出版日期2018-8