Mouse phenotyping

作者：Fuchs Helmut; Gailus Durner Valerie; Adler Thure; Aguilar Pimentel Juan Antonio; Becker Lore; Calzada Wack Julia; Da Silva Buttkus Patricia; Neff Frauke; Goetz Alexander; Hans Wolfgang; Hoelter Sabine M; Horsch Marion; Kastenmueller Gabi; Kemter Elisabeth; Lengger Christoph; Maier Holger; Matloka Mikolaj; Moeller Gabriele; Naton Beatrix; Prehn Cornelia; Puk Oliver; Racz Ildiko; Rathkolb Birgit; Roemisch Margl Werner; Rozman Jan; Wang Sattler Rui; Schrewe Anja

来源：Methods, 2011, 53(2): 120-135.

DOI：10.1016/j.ymeth.2010.08.006

Model organisms like the mouse are important tools to learn more about gene function in man. Within the last 20 years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in techniques, thus creating models which mimic human conditions. Due to pleiotropic effects, one gene may have different functions in different organ systems or time points during development. Therefore mutant mouse lines have to be phenotyped comprehensively in a highly standardized manner to enable the detection of phenotypes which might otherwise remain hidden. The German Mouse Clinic (GMC) has been established at the Helmholtz Zentrum Munchen as a phenotyping platform with open access to the scientific community (www.mousclinic.de; [1]). The GMC is a member of the EUMOD-IC consortium which created the European standard workflow EMPReSSslim for the systemic phenotyping of mouse models (http://www.eumodic.org/ [2]).

• 出版日期2011-2
• 单位河北医科大学