Gene profiling after knocking-down expression of nucleostemin in Hela cells using oligonucleotide DNA microarray

作者：Liu S J^*; Zhang Z H; Zhang D Q; Sui X M; Liu Y J; Cai Z W; Yuan X Y; Sun L Q; Hu G F; Liu R L

来源：Journal of Experimental & Clinical Cancer Research, 2006, 25(4): 575-583.

• nucleostemin
• Hela cells
• DNA microarray

Nucleostemin (NS) is preferentially and exclusively expressed in the stem cells and cancer cells, but not in differentiated adult tissues and cells. NS is likely to take part in controlling the proliferation and differentiation switch in stem cells and progenitor cells. Its deregulation in cancer also contributes to the elevated proliferation and undifferentiation of cancer cells. However, the mechanisms by which NS helps to maintain both cancer and stem cells in undifferentiated state remain unclear. In this study, we carried out gene profilings using oligonucleotide DNA microarray after knocking down the expression of NS in Hela cells. Of the 21,329 genes, 200 genes were found differentially expressed in NS silenced Hela cells with >2 fold ratio (either >2 or <0.5). Category analysis indicated these differential genes were mainly related with cancer pathogenesis, cell death, cell growth and proliferation. NS related gene pathway analysis suggested NS was mostly involved in the networks of cell cycle and differentiation controls. p53 may not be the only partner of NS in its regulated pathways. c-Myc may directly or indirectly interact with it to control the proliferation and differentiation switch in cancer cells. Our study provides a general view of the NS-target genes, and indicates the possible pathways in which NS plays its role in proliferation control.

• 出版日期2006-12
• 单位潍坊医学院; 北京积水潭医院; 中国科学院遗传与发育生物学研究所; 天津医科大学; 牡丹江医学院; 北京大学