Toll-like receptor 4 Asp299Gly and Thr399Ile polymorphisms: New data and a meta-analysis

作者：Senhaji Nezha^*; Diakit Brehima; Serbati Nadia; Zaid Younes; Badre Wafaa; Nadifi Sellama

来源：BMC Gastroenterology, 2014, 14(1): 206.

DOI：10.1186/s12876-014-0206-x

• Toll-like receptor 4
• Inflammatory bowel disease
• Moroccan patients

Background: The pathogenesis of inflammatory bowel disease (IBD) involves interactions between the host genetic susceptibility, intestinal microflora and mucosal immune responses through the pattern recognition receptor. Polymorphisms in toll-like receptor 4 (TLR4) induce an aberrant immune response to indigenous intestinal flora, which might favor IBD development. In this study, we aimed to determine whether TLR4 gene was associated with Crohn s disease (CD) and ulcerative colitis (UC) among Moroccan patients, and evaluated its correlation with clinical manifestation of the disease.
Methods: The study population comprised 117 patients with IBD and 112 healthy unrelated blood donors. TLR4 polymorphisms: Asp299Gly and Thr399Ile were genotyped by polymerase chain reaction-restriction fragment length polymorphism. PCR products were cleaved with Nco I for the Asp299Gly polymorphism and Hinf I for the Thr399Ile polymorphism. Meta-analysis was performed to test the association of 299Gly and 399Ileu carriage with CD, UC and the overall IBD risk.
Results: Our study revealed that the frequency of Asp299Gly and Thr399Ile did not differ significantly between patients and controls in the Moroccan population. However, meta-analysis demonstrated significantly higher frequencies of both Asp299Gly and Thr399Ile SNPs in IBD and CD and for 399Ileu carriage in UC patients.
Conclusion: The meta-analysis provides evidence that TLR4 polymorphisms confer a significant increased risk for the overall IBD development.

• 出版日期2014-12-10