Development and validation a liquid chromatography mass spectrometry for determination of solasodine in rat plasma and its application to a pharmacokinetic study

作者：Ma Jianshe; Ding Xitao; Sun Chengxiang; Lin Chongliang; An Xinxin; Lin Guanyang; Yang Xuezhi; Wang Xianqin^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014, 963: 24-28.

DOI：10.1016/j.jchromb.2014.05.031

• Solasodine
• LC-MS
• Pharmacokinetics
• Rat plasma

Solasodine is a poisonous alkaloid chemical compound that occurs in plants of the Solanaceae family. A simple and selective liquid chromatography mass spectrometry method for determination of solasodine in rat plasma was developed and validated over the range of 3-1000 ng/mL. Chromatographic separation was achieved on a C18 (2.1 mm x 50 mm, 3.5 mu m) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. The flow rate was set at 0.4 mL/min. After addition of midazolam as internal standard (IS), liquid-liquid extraction by ethyl acetate was used as sample preparation. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification with target ions m/z 414 for solasodine and m/z 326 for IS. Mean recoveries of solasodine in rat plasma were in the range of 87.6-94.1%. Matrix effects for solasodine were between 94.9% and 102.3%. Coefficient of variation of intra-day and inter-day precision were both <13%. The accuracy of the method ranged from 94.4% to 105.3%. The method was successfully applied to a pharmacokinetic study of solasodine after oral administration of 20 mg/kg in rats.

• 出版日期2014-7-15
• 单位温州医科大学