Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

作者：El Najjar N G; Farah M J; Hashwa F A; Tokajian S T^*

来源：Letters in Applied Microbiology, 2010, 51(4): 456-461.

DOI：10.1111/j.1472-765X.2010.02926.x

• antibiotic resistance
• class I integron
• RFLP
• UPEC
• variable region

Aim:
To study the prevalence and molecular basis of antimicrobial resistance in UPEC.
Methods and Results:
PCR was used to detect the presence of the Class I integron variable region (VR). The VR amplicons were then characterized by partial sequencing and restriction digestion with AluI. VR negative isolates showed more antibiotic susceptibility than VR positive isolates. 30% of the isolates were positive for the VR and carried the genes dfrA7, dfrA17-aadA5, dfrA1-aadA1, dfrA12-orf5-aadA2 and bla(OXA-30)-aadA1. Five restriction patterns were detected and isolates with the same VR amplicon size had the same restriction pattern.
Conclusions:
Our data demonstrated that Class I integrons are widely disseminated in Lebanon and showed their importance for the occurrence and transmission of multidrug resistance.
Significance and Impact of the Study:
These findings will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in UPEC.

• 出版日期2010-10