A new criteria for screening macroprolactinemia using polyethylene glycol treatment combined with different assays for prolactin

作者：Chen, Y. -J.^*; Song, G. -Z.; Wang, Z. -N.

来源：European Review for Medical and Pharmacological Sciences, 2016, 20(9): 1788-1794.

• Polyethylene glycols/diagnostic
• Hyperprolactinemia/diagnosis
• Prolactin
• Macroprolactin
• Gel filtration chromatography

OBJECTIVE: To establish the criteria for screening macroprolactinemia (MP) after Polyethylene glycol (PEG) treatment plus different assays of Prolactin (PRL) in a clinical setting.
PATIENTS AND METHODS: This study recruited 122 patients, and their PRL-elevated serum samples were collected. Twenty-two of the 122 serum samples were treated by gel filtration chromatography (GFC) and PEG precipitation. The PRL content in the eluent and supernatant were tested separately by two analyzers: i2000sr (Abbott Laboratories, Chicago, IL, USA) and E170 (Roche Diagnostics, Basel, Switzerland). The GFC-method (the gold standard) was applied, in order to establish the criteria of screening MP after PEG-method. The MP positive rate and PRL concentrations in 100 PRL-elevated patients were compared between the literature criteria (a PRL recovery of < 40%) and the new criteria.
RESULTS: The detected value of macroprolactin with the new criteria in the i2000sr (a PRL recovery of < 50%) was higher than in the E170 (a PRL recovery of < 60%). The E170 analyzer detected 38 cases of MP in 100 PRL-elevated samples by using new criteria, which was higher than the literature criteria (24 cases) (p < 0.05). Therefore, there were 15 samples could not be judged consistently using the literature criteria by the i2000sr and E170 analyzers. There was also a significant difference in PRL concentrations between the two groups (p < 0.01). If using the new criteria, the inconsistency between the two analyzers was reduced to 2 samples. There was a very significant difference in the inconsistency between two criteria (p < 0.01). The reported values of PRL from the i2000sr and E170 were 27.8 (16.2-42.6) ng/ml and 32.0 (19.6-49.9) ng/ml. There were no significant differences between two analyzers.
CONCLUSIONS: Establishment of different criteria for screening MP by using PEG-method is helpful for the accuracy of PRL determination and its comparability.

• 出版日期2016-5
• 单位杭州医学院; 浙江省人民医院