A Fast Chromatographic Method for Determination of Daidzein and Genistein in Spiked Water River Samples Using Multivariate Curve Resolution

作者：Moreira Edilene D T; Pinto Licarion; Gomes Adriano A; Goicoechea Hector C^*; Araujo Mario C U

来源：Journal of the Brazilian Chemical Society, 2015, 26(8): 1573-1582.

DOI：10.5935/0103-5053.20150125

• emerging contaminants
• liquid chromatography
• multiway calibration
• second order advantage

This work reports the development of a fast chromatographic methodology for quantitation of two phytoestrogens: daidzein (DAI), and genistein (GEN), in river water samples. The proposed method is based on high performance liquid chromatography-diode array detection (HPLC-DAD) data, and multivariate curve resolution-alternative least square (MCR-ALS) second-order calibration. Initially, the method was evaluated analyzing a synthetic validation set; prepared based on a Taguchi design. Subsequently, the method was applied to predict the concentration of the phytoestrogens in spiked river water samples, previously pre-processed by solid phase extraction (SPE). By implementation of the present chromatographic methodology, a 50% reduction in operation time was achieved (from 7.00 to 3.25 min) when compared with previous work in the literature. Precision was achieved even in the presence of non-modeled constituents and strong background. Thus, the proposed method is a rapid and robust alternative for the quantitation of studied phytoestrogens.

• 出版日期2015-8