Second order advantage in the determination of amaranth, sunset yellow FCF and tartrazine by UV-vis and multivariate curve resolution-alternating least squares

作者：Elizabeth Llamas Natalia; Garrido Mariano; Susana Di Nezio Maria; Fernandez Band Beatriz Susana

来源：Analytica Chimica Acta, 2009, 655(1-2): 38-42.

DOI：10.1016/j.aca.2009.10.001

• Multivariate curve resolution
• Alternating least squares
• Second order advantage
• Dyes
• Beverages samples

A direct spectrophotometric method for the determination of three artificial colors - amaranth, sunset yellow FCF and tartrazine - in beverages samples is proposed. The spectra were recorded between 359 and 600 nm. The spectra of the samples (just filtrated), pure dyes (concentrations ranged between 0.01 and 1.8 mg L(-1) for amaranth, 0.08 and 4.4 mg L(-1) for sunset yellow and 0.04 and 1.8 mg L(-1) for tartrazine) and synthetic mixtures were disposed in a column-wise augmented data matrix. This kind of data structure, analyzed by multivariate curve resolution-alternating least squares (MCR-ALS) makes it possible to exploit the so called 'second order advantage'. MCR-ALS algorithm was applied to the experimental data under the non-negativity and equality constraints. As a result, the concentration of each dye in the samples and their corresponding pure spectra were obtained.
The results were validated using internal reference materials and no significant differences were found (alpha = 5%) between the reference values and the ones obtained with the proposed method. The second order advantage made it possible to obtain unbiased results even in the presence of interferences. m 2009 Elsevier B.V.

• 出版日期2009-11-23