A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry

作者：Lu Jin; Ren Xue; Liu Xiang-Liang; Liu Ze-Feng^*

来源：Chinese Journal of Analytical Chemistry, 2018, 46(1): 67-73.

DOI：10.11895/j.issn.0253.3820.171412

• Cervical cancer
• Ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry
• Metabonomics
• Urine
• Biomarkers

This work aims to analyze and compare the potential biomarkers between cervical cancer (CC) patients and healthy controls (HC). The urine samples of 11 CC patients (age (45. 65 +/- 5.6) years) and 11 HC patients (age (45. 9 +/- 3. 2) years) were collected. The metabolites of urine samples from CC and HC were analyzed by using ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap MS/MS), which could provide evidence for early diagnose and disease pathway. The LC-MS data of urines were analyzed by principal components analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to identify the potential biomarkers. Urine samples of CC patients were successfully discirminated from those of healthy controls. A total of twelve significant metabolites were found and identified as potential biomarkers according to the established UHPLC-Q-Orbitrap MS and MS/MS method. Identification of biomarkers between CC and HC patients may play an important role in the study of mechanism of UC and its pathway.

• 出版日期2018-1
• 单位吉林大学