Nanobarcoded Superparamagnetic Iron Oxide Nanoparticles for Nanomedicine: Quantitative Studies of Cell-Nanoparticle Interactions by Scanning Image Cytometry

作者：Eustaquio Trisha; Leary James F^*

来源：Cytometry, Part A, 2016, 89A(2): 207-216.

DOI：10.1002/cyto.a.22699

• nanoparticles
• image analysis
• reactive oxygen species
• nanotoxicity
• apoptosis
• necrosis

Oligonucleotide-functionalized nanoparticles (NPs) are promising agents for nanomedicine, but the potential in vitro nanotoxicity that may arise from such conjugates has yet to be evaluated in a dose response manner. Since nanomedicine functions on the single-cell level, measurements of nanotoxicity should also be performed as such. In vitro single-cell nanotoxicity assays based on scanning image cytometry are used to study a specific type of oligo-functionalized NP, "nanobarcoded" superparamagnetic iron oxide NPs (NB-SPIONs). The selected panel of single-cell assays measures well-known modes of nanotoxicity-apoptosis, necrosis, generation of reactive oxygen species (ROS), and cell number. Using these assays, the cytotoxicity of two sizes of NB-SPIONs (10 nm and 30 nm core size) was compared to the parent NP, carboxylated SPIONs (COOH-SPIONs). The results suggest that the conjugated NB confers a biocompatible coating that protects against cytotoxicity at very high SPION doses, but both NB-and COOH-SPIONs of either size generally have low in vitro cytotoxicity at physiologically relevant doses.

• 出版日期2016-2