A male-specific QTL for social interaction behavior in mice mapped with automated pattern detection by a hidden Markov model incorporated into newly developed freeware

作者：Arakawa Toshiya; Tanaveh Akira; Ikeuchi Shiho; Takahashi Aki; Kakihara Satoshi; Kimura Shingo; Sugimoto Hiroki; Asada Nobuhiko; Shiroishi Toshihiko; Tomihara Kazuya; Tsuchiya Takashi; Koide Tsuyoshi^*

来源：Journal of Neuroscience Methods, 2014, 234: 127-134.

DOI：10.1016/j.jneumeth.2014.04.012

• Social interaction
• Mouse
• Consomic strain

Background: Owing to their complex nature, social interaction tests normally require the observation of video data by a human researcher, and thus are difficult to use in large-scale studies. We previously established a statistical method, a hidden Markov model (HMM), which enables the differentiation of two social states (%26quot;interaction%26quot; and %26quot;indifference%26quot;), and three social states (%26quot;sniffing%26quot;, %26quot;following%26quot;, and %26quot;indifference%26quot;), automatically in silico. %26lt;br%26gt;New method: Here, we developed freeware called DuoMouse for the rapid evaluation of social interaction behavior. This software incorporates five steps: (1) settings, (2) video recording, (3) tracking from the video data, (4) HMM analysis, and (5) visualization of the results. %26lt;br%26gt;Results: Using DuoMouse, we mapped a genetic locus related to social interaction. We previously reported that a consomic strain, B6-Chr6C(MSM), with its chromosome 6 substituted for one from MSM/Ms, showed more social interaction than C57BL/6 (B6). We made four subconsomic strains, C3, C5, C6, and C7, each of which has a shorter segment of chromosome 6 derived from B6-Chr6C, and conducted social interaction tests on these strains. DuoMouse indicated that C6, but not C3, C5, and C7, showed higher interaction, sniffing, and following than B6, specifically in males. %26lt;br%26gt;Comparison with existing method: The data obtained by human observation showed high concordance to those from DuoMouse. The results indicated that the MSM-derived chromosomal region present in C6 but not in C3, C5, and C7-associated with increased social behavior. %26lt;br%26gt;Conclusions: This method to analyze social interaction will aid primary screening for difference in social behavior in mice.

• 出版日期2014-8-30