Development of a liquid chromatography-mass spectrometry method for determination of agrimol B in rat plasma: application to preclinical pharmacokinetics

作者：Wang, Zhenglin^*; Liu, Peng; Liang, Pin; Hu, Xiang

来源：Biomedical Chromatography, 2015, 29(4): 481-484.

DOI：10.1002/bmc.3299

• Agrimonia pilosa Ledeb
• agrimol B
• LC-MS
• rat plasma
• pharmacokinetics

A sensitive and accurate liquid chromatography coupled with mass spectrometry (LC-MS) method was developed for the determination of agrimol B, a main active ingredient isolated from Agrimonia pilosa Ledeb., in rat plasma. Chromatographic separation was achieved on a Zorbax CN column (150x4.6mm, 5 mu m), with isocratic elution consisting of acetonitrile and water (15:85, v/v) at a flow rate of 0.6mL/min. Agrimol B and dryocrassin ABBA, an internal standard (IS), were analyzed by selected ion monitoring at m/z transitions of 681.3 and 819.4, respectively. This assay exhibited a good linearity with a correlation coefficient >0.99 and showed no endogenous interference with the analyte and IS. The limit of quantification of agrimol B was 8.025ng/mL with acceptable precision and accuracy. The method was successfully applied in the pharmacokinetic study of agrimol B in rats after intravenous (1mg/kg) and oral (2, 5 and 10mg/kg) doses of agrimol B. The absolute bioavailability of agrimol B was 16.4-18.0% in rat. Our study clarifies the pharmacokinetic behavior of agrimol B in animals.

• 出版日期2015-4
• 单位大连医科大学