Adipokine Gene Single-Nucleotide Polymorphisms in Portuguese Obese Adolescents: Associations with Plasma Concentrations of Adiponectin, Resistin, IL-6, IL-1, and TNF-

作者：Nascimento Henrique^*; Vieira Emilia; Coimbra Susana; Catarino Cristina; Costa Elisio; Bronze da Rocha Elsa; Rocha Pereira Petronila; Carvalho Marcia; Mansilha Helena Ferreira; Rego Carla; dos Santos Rosario; Santos Silva Alice; Belo Luis^*

来源：Childhood Obesity, 2016, 12(4): 300-313.

DOI：10.1089/chi.2015.0235

Background: The genetic contribution to obesity and to circulating adipokine levels has not been completely clarified. We aimed to evaluate adipokine genes' single-nucleotide polymorphism (SNP) prevalence and its association with circulating adipokine levels and risk factors for cardiovascular disease in an obese Portuguese pediatric population. Methods: Two hundred forty-eight obese adolescents (mean age 13.4 years old; 47.2% females) participated in a cohort study. We screened 12 SNPs by direct sequencing in five adipokine genes: adiponectin (ADIPOQ: rs16861194, rs17300539, rs266729, rs2241766, rs1501299), interleukin-1 (IL-1; rs1143627), IL-6 (IL-6; rs1800795), tumor necrosis factor- (TNF-; rs1800629), and resistin (RETN; rs1862513, rs3219177, rs3745367, rs3745368). Biochemical analysis included determination of circulating adipokines, C-reactive protein (CRP) levels, lipid profile, and markers of insulin resistance. Results: Compared to males, females presented higher circulating levels of insulin, adiponectin, IL-6, resistin, and leptin concentrations, but lower TNF- levels. No statistically significant differences were found for genotype or allelic distributions between genders. In the whole sample population, adiponectin levels were influenced by ADIPOQ rs17300539 (c.-1138G>A; lower in subjects with GG genotype). When only males were considered, IL-1, IL-6, and TNF- levels were associated with ADIPOQ rs1501299 (c.214+62G>T; higher in GG subjects). TNF- concentrations were modulated by TNF-rs1800629 (c.-488G>A; lower in GG males), RETN rs1862513 (c.-216C>G; higher in CC subjects), and RETN rs3219177 (c.118+39C>T; higher in CC subjects). Leptin levels were influenced by IL-1 rs1143627 (c.-118C>T) presenting TT individuals' lower levels. Conclusions: Our data demonstrate that in pediatric obese patients, some adipokine gene SNPs have an association with circulating adipokine levels and lipid profile.

• 出版日期2016-8