Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer)

作者：Goosen Wynand J; Miller Michele A; Chegou Novel N; Cooper David; Warren Robin M; van Helden Paul D; Parsons Sven D C^*

来源：Veterinary Immunology and Immunopathology, 2014, 160(1-2): 133-138.

DOI：10.1016/j.vetimm.2014.03.015

• African buffalo
• Bovigam (R)
• Bovine tuberculosis
• CFP-10
• ESAT-6
• Interferon-gamma

We assessed the use of Mycobacterium bovis-specific peptides for the diagnosis of tuberculosis in African buffaloes (Syncenis coffer) by evaluating the agreement between the single intradermal comparative tuberculin test (SICTT), the Bovigam (R) EC (BEC) assay, the Bovigam (R) HP (BHP) assay and two assays utilizing the QuantiFERON (R) TB-Gold (in tube) system employing 20 h (mQFT20 assay) and 30 h (mQFT30 assay) whole blood incubation periods. Of 84 buffaloes, 45% were SICTT-positive, 48% were BEC-positive, 50% were BHP-positive, 37% were mQFT20-positive and 43% were mQFT30-positive. Agreement between the BEC and BHP Bovigam (R) assays was high (kappa = 0.86,95% CI 0.75-0.97) and these detected the most test-positive animals suggesting that they were the most sensitive assays. Interferon-gamma release was significantly greater in buffaloes that were test-positive for all tests than in animals with discordant but positive Bovigam (R) results. Agreement between the mQFT assays was equally high (kappa = 0.88,95% CI 0.77-0.98); however, all buffaloes with discordant mQFT results (n = 6) were mQFT30-positive/mQFT20-negative, including three confirmed M. bovis-infected animals, suggesting that the mQFT30 assay is the more sensitive of the two. Agreements between the two Bovigam (R) and two mQFT assays were moderate, suggesting that in its current format the mQFT assay is less sensitive than either the BEC or the BHP assays.

• 出版日期2014-7-15