Aflatoxin M-1 in UHT milk consumed in Turkey and first assessment of its bioaccessibility using an in vitro digestion model

作者：Kabak Bulent^*; Ozbey Fatih

来源：Food Control, 2012, 28(2): 338-344.

DOI：10.1016/j.foodcont.2012.05.029

• Aflatoxin M-1
• UHT milk
• Occurrence
• Bioaccessibility
• HPLC
• Daily intake

Contamination of milk and dairy products with aflatoxin M-1 (AFM(1)) continues to receive increased attention because of its potential health hazard to humans. The first aim of this study was to know the occurrence and levels of AFM(1) in whole UHT milk from main processors in Turkey in order to make a preliminary exposure assessment. A total of 40 milk samples were analysed for AFM(1) using high performance liquid chromatography with fluorescence detection (HPLC-FD) after immunoaffinity column clean-up. Aflatoxin M1 was detected in 20% of samples at levels ranging from <0.004 to 0.076 mu g l(-1). Only two samples contained AFM(1) above the EU limit of 0.05 mu g l(-1)
The second aim of this study was to determine the bioaccessibility of AFM(1) from milk using an in vitro digestion model. The bioaccessibility of AFM(1) in spiked and naturally contaminated milk samples ranged from 80.5 to 83.8% and from 81.7 to 86.3%, respectively. No difference (P > 0.05) in AFM(1) bioaccessibility was found between spiked and naturally contaminated milk samples. This study also assessed the binding of AFM(1) by six probiotic bacteria under simulated gastrointestinal conditions. A 15.5-31.6% reduction in AFM(1) bioaccessibility was observed in the presence of probiotic bacteria. Based on the results obtained in the present study, the mean daily intake of AFM(1) through milk consumption was estimated as 0.008 ng kg(-1) b.w. day(-1) for Turkish adults.

• 出版日期2012-12