Nanoscopic cell-wall architecture of an immunogenic ligand in Candida albicans during antifungal drug treatment

作者：Lin Jia; Wester Michael J; Graus Matthew S; Lidke Keith A; Neumann Aaron K

来源：Molecular Biology of the Cell, 2016, 27(6): 1002-1014.

DOI：10.1091/mbc.E15-06-0355

The cell wall of Candida albicans is composed largely of polysaccharides. Here we focus on beta-glucan, an immunogenic cell-wall polysaccharide whose surface exposure is often restricted, or "masked," from immune recognition by Dectin-1 on dendritic cells (DCs) and other innate immune cells. Previous research suggested that the physical presentation geometry of beta-glucan might determine whether it can be recognized by Dectin-1. We used direct stochastic optical reconstruction microscopy to explore the fine structure of beta-glucan exposed on C. albicans cell walls before and after treatment with the antimycotic drug caspofungin, which alters glucan exposure. Most surface-accessible glucan on C. albicans yeast and hyphae is limited to isolated Dectin-1-binding sites. Caspofungin-induced unmasking caused approximately fourfold to sevenfold increase in total glucan exposure, accompanied by increased phagocytosis efficiency of DCs for unmasked yeasts. Nanoscopic imaging of caspofungin-unmasked C. albicans cell walls revealed that the increase in glucan exposure is due to increased density of glucan exposures and increased multiglucan exposure sizes. These findings reveal that glucan exhibits significant nanostructure, which is a previously unknown physical component of the host-Candida interaction that might change during antifungal chemotherapy and affect innate immune activation.

• 出版日期2016-3-15