Acrolein induces a cellular stress response and triggers mitochondrial apoptosis in A549 cells

作者：Roy Julie; Pallepati Pragathi; Bettaieb Ahmed; Tanel Andre; Averill Bates Diana A^*

来源：Chemico-Biological Interactions, 2009, 181(2): 154-167.

DOI：10.1016/j.cbi.2009.07.001

• Acrolein
• Apoptosis
• Mitochondria
• AKT
• Inhibitor of apoptosis protein
• Oxidative stress

Acrolein is a highly reactive, alpha,beta-unsaturated aldehyde that is an omnipresent environmental pollutant. Humans are exposed to acrolein in food, vapors of overheated cooking oil, cigarette smoke and by combustion of organic products. Acrolein is a toxic by-product of lipid peroxidation resulting from oxidative stress, which is implicated in pulmonary, cardiac and neurodegenerative diseases. Low dose exposure to toxic compounds often leads to adaptive responses. if the adaptive response does not counteract the adverse exposure, death processes such as apoptosis will eliminate the cell. This study investigates the activation of antiapoptosis survival factors in relation to the induction of cell death by apoptosis, following exposure to low doses of acrolein, in A549 human lung cells. Exposure to acrolein (<15 mu M, 30 min) activated the survival factor AKT, which led to phosphorylation of Bad and induction of antiapoptosis proteins clAP1/2. Acrolein (10-50 mu M, 30-60 min) increased reactive oxygen species and caused mitochondrial membrane hyperpolarisation. Inhibition by the antioxidants catalase, polyethylene glycol-catalase, sodium pyruvate and MnTBAP showed that acrolein-induced reactive oxygen species were responsible for mitochondrial membrane hyperpolarisation. Acrolein (3-27 mu M, 30-60 min) activated early stage processes in the mitochondrial pathway of apoptosis, such as Bax translocation to mitochondria, cytochrome c release, caspase-9 activation, and translocation of apoptosis-inducing factor to the nucleus. Acrolein (10-50 mu M) triggered later stage processes such as activation of caspases-3, -7 and -6, phosphatidylserine externalization and cleavage of poly(ADP)ribose polymerase after longer times (2h). These events were inhibited by polyethylene glycol-catalase, showing that apoptosis was mediated by overproduction of reactive oxygen species by acrolein. The novel findings show that antiapoptosis processes dominate at low dose (<15 mu M)/shorter exposure times to acrolein, whereas proapoptotic processes dominate at higher dose (10-50 mu M)/longer exposure times. Acrolein induced apoptosis through the mitochondrial pathway that was mediated by reactive oxygen species.

• 出版日期2009-10-7