Association of ADAMTS-7 Levels with Cardiac Function in a Rat Model of Acute Myocardial Infarction

作者：Wu, Wenjing; Wang, Hui; Yu, Changan; Li, Jiahui; Gao, Yanxiang; Ke, Yuannan; Wang, Yong; Zhou, Yifeng^*; Zheng, Jingang^*

来源：Cellular Physiology and Biochemistry, 2016, 38(3): 950-958.

DOI：10.1159/000443047

• ADAMTS-7
• Acute myocardial infarction
• Cardiac function
• Cartilage oligomeric matrix protein

Background/Aims: High ADAMTS-7 levels are associated with acute myocardial infarction (AMI), although its involvement in ventricular remodeling is unclear. In this study, we investigated the association between ADAMTS-7 expression and cardiac function in a rat AMI model. Methods: Sprague-Dawley rats were randomized into AMI (n = 40) and sham (n = 20) groups. The left anterior descending artery was sutured to model AMI. Before surgery and 7, 14, 28, and 42 days post-surgery, ADAMTS-7 and brain natriuretic peptide (BNP), and cartilage oligomeric matrix protein (COMP) were assessed by ELISA, western blot, real-time RT-PCR, and/or immunohistochemistry. Cardiac functional and structural parameters were assessed by M-mode echocardiography. Results: After AMI, plasma ADAMTS-7 levels increased, peaking on day 28 (AMI: 13.2 +/- 6.3 vs. sham: 3.4 +/- 1.3 ng/ml, P < 0.05). Compared with the sham group, ADAMTS-7 expression was higher in the infarct zone at day 28. COMP present in normal myocardium was degraded by day 28 post-AMI. Plasma ADAMTS-7 correlated positively with BNP (r = 0.642, P = 0.025), left ventricular end-diastolic diameter (r = 0.695, P = 0.041), left ventricular end-systolic diameter (r = 0.710, P = 0.039), left ventricular ejection fraction (r = 0.695, P = 0.036), and left ventricular short-axis fractional shortening (r = 0.721, P = 0.024). Conclusions: ADAMTS-7 levels may reflect the degree of ventricular remodeling after AMI.

• 出版日期2016
• 单位中日友好医院