Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes

作者：Eng George; Lee Benjamin W; Protas Lev; Gagliardi Mark; Brown Kristy; Kass Robert S; Keller Gordon; Robinson Richard B; Vunjak Novakovic Gordana^*

来源：Nature Communications, 2016, 7(1): 10312.

DOI：10.1038/ncomms10312

The therapeutic success of human stem cell-derived cardiomyocytes critically depends on their ability to respond to and integrate with the surrounding electromechanical environment. Currently, the immaturity of human cardiomyocytes derived from stem cells limits their utility for regenerative medicine and biological research. We hypothesize that biomimetic electrical signals regulate the intrinsic beating properties of cardiomyocytes. Here we show that electrical conditioning of human stem cell-derived cardiomyocytes in three-dimensional culture promotes cardiomyocyte maturation, alters their automaticity and enhances connexin expression. Cardiomyocytes adapt their autonomous beating rate to the frequency at which they were stimulated, an effect mediated by the emergence of a rapidly depolarizing cell population, and the expression of hERG. This rate-adaptive behaviour is long lasting and transferable to the surrounding cardiomyocytes. Thus, electrical conditioning may be used to promote cardiomyocyte maturation and establish their automaticity, with implications for cell-based reduction of arrhythmia during heart regeneration.

• 出版日期2016-1