An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker

作者：Jin Kai; Zhang Yongjun; Luo Zhibing; Xiao Yuehua; Fan Yanhua; Wu Di; Pei Yan^*

来源：Biotechnology Letters, 2008, 30(8): 1379-1383.

DOI：10.1007/s10529-008-9713-6

• Beauveria bassiana
• fusion gene
• green fluorescent protein
• phosphinothricin resistance
• transformation

An improved transformation method for the biocontrol agent, Beauveria bassiana, was developed. For convenience of transformation selection and detection, the coding regions of the genes for phosphinothricin acetlytransferase and green fluorescent protein were fused and an expression vector, pBFT, carrying this fusion was constructed. Under optimum conditions, over 60 transformants mu g(-1) plasmid DNA were obtained. B. bassiana conidia frozen 1 month at -80 degrees C were fully competent for transformation. The method was significantly less laborious and more rapid than current methods for B. bassiana. The bar::egfp provides a selectable and visible marker which may expedite future genetic engineering of this fungus.

• 出版日期2008-8
• 单位西南大学