A novel mutation in the cathepsin C gene in a Pakistani family with Papillon-Lefevre syndrome

作者：Kurban M; Cheng T; Wajid M; Kiuru M; Shimomura Y; Christiano A M^*

来源：Journal of the European Academy of Dermatology and Venereology, 2010, 24(8): 967-969.

DOI：10.1111/j.1468-3083.2009.03575.x

• cathepsin C
• gingivitis
• palmoplantar keratoderma
• Papillon-Lefevre syndrome

Background
Papillon-Lefevre syndrome (PLS; OMlM 245000) is an autosomal recessive disease caused by mutations in cathepsin C (CTSC) gene and is characterized by palmoplantar keratoderma, psoriasiform lesion over the extensor surfaces and gingivitis followed by loss of teeth. CTSC gene is expressed in several tissues including the skin and cells of the immune system. In the skin, CTSC plays a role in differentiation and desquamation, whereas in the immune system, it activates serine proteases.
Objectives
We analysed the molecular basis of PLS in a Pakistani family.
Methods
Genomic DNA was isolated from the sample according to standard techniques. All exons of the CTSC gene with adjacent sequences of exon-intron borders were amplified by PCR and directly sequenced.
Results
We identified a novel deletion mutation designated c.2ldelG (Leu7PhefsX57) in exon 1 of the CTSC gene, which probably results in the absence of CTSC protein.
Conclusion
Our data further expand the spectrum of mutations in the CTSC gene underlying PLS.

• 出版日期2010-8