Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5

作者：Basit Hajra^*; Sharma K Shivaji; Van der Heyden Angeline; Gondran Chantal; Breyton Cecile; Dumy Pascal; Winnik Francoise M; Labbe Pierre

来源：Chemical Communications, 2012, 48(48): 6037-6039.

DOI：10.1039/c2cc31107k

Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.

• 出版日期2012
• 单位中国地震局