Digital holographic microscopy long-term and real-time monitoring of cell division and changes under simulated zero gravity

作者：Pan, Feng^*; Liu, Shuo; Wang, Zhe; Shang, Peng; Xiao, Wen

来源：Optics Express, 2012, 20(10): 11496-11505.

DOI：10.1364/OE.20.011496

• REFRACTIVE-INDEX
• LIVING CELLS
• MORPHOMETRY
• ENVIRONMENT
• SPACE

The long-term and real-time monitoring the cell division and changes of osteoblasts under simulated zero gravity condition were succeed by combing a digital holographic microscopy (DHM) with a superconducting magnet (SM). The SM could generate different magnetic force fields in a cylindrical cavity, where the gravitational force of biological samples could be canceled at a special gravity position by a high magnetic force. Therefore the specimens were levitated and in a simulated zero gravity environment. The DHM was modified to fit with SM by using single mode optical fibers and a vertically-configured jig designed to hold specimens and integrate optical device in the magnet's bore. The results presented the first-phase images of living cells undergoing dynamic divisions and changes under simulated zero gravity environment for a period of 10 hours. The experiments demonstrated that the SM-compatible DHM setup could provide a highly efficient and versatile method for research on the effects of microgravity on biological samples.

• 出版日期2012-5-7
• 单位西北工业大学; 北京航空航天大学