Bisphenol A alters the cardiovascular response to hypoxia in Danio rerio embryos

作者：Cypher Alysha D^*; Ickes Jessica R; Bagatto Brian

来源：Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 2015, 174: 39-45.

DOI：10.1016/j.cbpc.2015.06.006

• Endocrine disruption
• Bisphenol A
• Hypoxia
• Zebrafish
• Cardiovascular
• Co-exposure
• HIF-1 alpha

The purpose of this study was to determine if the cardiovascular response to hypoxia was altered by the presence of bisphenol A (BPA) in Danio rerio embryos. It was expected that BPA exposure would affect cardiovascular parameters during hypoxia more than normoxia due to an interaction between BPA and the hypoxia-inducible factor (HIF-1 alpha) pathway. We demonstrate that BPA exposure has a minimal effect during normoxia but can severely affect the cardiovascular system during a hypoxic event. Cardiovascular response was measured in vivo using video microscopy and digital motion analysis. RBC density increased 35% in hypoxia alone but decreased 48% with addition of 0.25 mg/L BPA. Tissue vascularization (% coverage) was unaffected by hypoxia alone but decreased 37% with addition of 025 mg/L BPA. The diameter and RBC velocity of arteries were more sensitive than veins to BPA exposure during both normoxia and hypoxia. Arterial RBC velocity decreased 42% during normoxia and 52% during hypoxia with 1 mg/L BPA. This decrease in velocity may in part be due to the 86% decrease in heart rate (f(H)) observed during co-exposure to hypoxia and 5 mg/L BPA. While stroke volume (S-V) was unaffected by treatment, cardiac output (Q) decreased by 69% with co-exposure. f(H) and Q were not affected by BPA exposure during normoxia. Development ultimately slowed by 146% and mortality rates were 95% during hypoxia when exposed to 5 mg/L BPA. Our results show for the first time that BPA exposure alters the cardiovascular system during hypoxia more so than normoxia.

• 出版日期2015-9