Association of phthalate exposures with urinary free cortisol and 8-hydroxy-2 '-deoxyguanosine in early childhood

作者：Kim Jin Hee; Lee Jangwoo; Moon Hyo Bang; Park Jeongim; Choi Kyungho; Kim Sung Koo^*; Kim Sungkyoon^*

来源：Science of the Total Environment, 2018, 627: 506-513.

DOI：10.1016/j.scitotenv.2018.01.125

• Phthalate metabolites
• Free cortisol
• 8-Hydroxy-2 '-deoxyguanosine (8-OHdG)
• Mother-child pairs

Several studies suggested potential links of phthalates to stress-related outcomes. However, limited evidence has been available for the relationships between phthalate metabolites and free cortisol and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in perinatal and postnatal environments. Therefore, we evaluated the relationships between phthalate metabolites and free cortisol and 8-OHdG in mother-child pairs. We repeatedly collected urine samples of 287 mother-child pairs from just before delivery to 15 months of age to measure the levels of four phthalate metabolites - mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP), mono-isobutyl phthalate (MiBP), and mono-n-butyl phthalate (MnBP) - and free cortisol and 8-OHdG. We used linear mixed effect models and generalized additive mixed models to estimate the relationship between the phthalate metabolites and free cortisol and 8-OHdG after adjusting for the child's gender, urine collection time, and maternal smoking status. The four phthalate metabolite levels were strongly correlated each other (all, p < .0001), and intra-class correlation for each metabolite in children ranged from 0.18 to 0.96. All four phthalate metabolites were positively associated with both free cortisol (MEHHP, beta = 0.18 and p < .0001; MEOHP, beta=0.17 and p < .0001; MiBP, beta = 0.13 and p=.0001; MnBP, beta = 0.21 and p < .0001; and molar sum of metabolites, beta=0.21 and p < .0001) and 8-OHdG ( MEHHP, beta=0.20 and p<.0001; MEOHP, beta=0.18 and p<.0001; MiBP, beta=0.23 and p<.0001; MnBP, beta=0.28 and p<.0001; and molar sum of metabolites, beta = 0.29 and p<.0001) in childhood. Our findings suggest that phthalate exposures increase free cortisol and 8-OHdG levels in early childhood.

• 出版日期2018-6-15