Adenosine Deaminase Acts as a Natural Antagonist for Dipeptidyl Peptidase 4-Mediated Entry of the Middle East Respiratory Syndrome Coronavirus

作者：Raj V Stalin; Smits Saskia L; Provacia Lisette B; van den Brand Judith M A; Wiersma Lidewij; Ouwendijk Werner J D; Bestebroer Theo M; Spronken Monique I; van Amerongen Geert; Rottier Peter J M; Fouchier Ron A M; Bosch Berend Jan; Osterhaus Albert D M E; Haagmans Bart L^*

来源：Journal of Virology, 2014, 88(3): 1834-1838.

DOI：10.1128/JVI.02935-13

Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection.

• 出版日期2014-2