A Rapid Assay for the Simultaneous Determination of Nicotine, Cocaine and Metabolites in Meconium Using Disposable Pipette Extraction and Gas ChromatographyMass Spectrometry (GCMS)

作者：Mozaner Bordin Dayanne C; Alves Marcela N R; Cabrices Oscar G; de Campos Eduardo G; De Martinis Bruno Spinosa

来源：Journal of Analytical Toxicology, 2014, 38(1): 31-38.

DOI：10.1093/jat/bkt092

Drug abuse by pregnant women is considered a serious public health problem worldwide. Meconium is the first excretion in newborns and has been used as an alternative matrix to evaluate in utero drug exposure. Solid phase extraction (SPE) is widely employed to prepare and clean up samples in the field of forensic analysis. Most SPE products require large volumes of solvent, which culminates in longer sample processing times and increased cost per sample. Disposable pipette extraction (DPX) tips have been used as an alternative to traditional SPE cartridges. They combine efficient and rapid extraction with reduced solvent consumption. The purpose of this study was to develop and validate a method to determine nicotine, cotinine, cocaine, benzoylecgonine, cocaethylene and methyl ester anhydroecgonine in meconium using DPX and gas chromatographymass spectrometry (GCMS). Validation results indicated that extraction efficiency ranged 5098, accuracy 92106, intra-assay precision 412 and inter-assay precision 612. Linear calibration curves resulted in R-2 values 0.99, limits of detection ranged from 2.5 to 15 ng/g and the limit of quantitation from 10 to 20 ng/g. The DPXGCMS method was shown to selectively analyze trace concentrations of drugs in meconium samples. Finally, the developed and validated method was applied to 50 meconium samples.

• 出版日期2014-2