A single amino acid substitution converts gamma-glutamyltranspeptidase to a class IV cephalosporin acylase (glutaryl-7-aminocephalosporanic acid acylase)

作者：Suzuki H^*; Miwa C; Ishihara S; Kumagai H

来源：Applied and Environmental Microbiology, 2004, 70(10): 6324-6328.

DOI：10.1128/AEM.70.10.6324-6328.2004

The aspartyl residue at position 433 of gamma-glutamyltranspeptidase of Escherichia coli K-12 was replaced by an asparaginyl residue. This substitution enabled gamma-glutamyltranspeptidase to deacylate glutaryl-7-aminocephalosporanic acid, producing 7-aminocephalosporanic acid, which is a starting material for the synthesis of semisynthetic cephalosporins.

• 出版日期2004-10